Boston Micromedia Manual
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Deformable Medium Technologies, and Elasticsearch and Synthace , teamed up for an open ended real-world project to more efficiently and cheaply manufacture deformable materials. The project aimed to generate a database of standard test conditions, based on a visualization of material mechanics. This enabled to perform iterative experiments that were repeatable and accurate, and which could be translated to manufacturing. The automation of these steps enabled a large number of samples to be processed quickly.
On this manual, we will re-show the first case study of efforts similar to the ill-fated Boston bionics project, which aims to develop a transparent, 3D printed brain organ on a desktop via an extrusion-based 3D bioprinter. In the Boston bionics manual, we will show the first efforts to replace a standard cover slip with a transparent, thickest plasticized agarose gel (3% agarose) to facilitate outstanding imaging of neurons, with a deformable hydrogel. We will investigate several extruder head configurations, together with different liquid bed thickness, to achieve the highest agarose gel extrusion speed. In addition, we will report a study that implemented a repulsive photopolymer that does not adhere to the extruded agarose, representing the first-ever extrusion-based bioprinting in situ, and patching studies of neurons using soft hydrogels. Interestingly, this modular work open-endedly and aims to stimulate a real-world application of the material, thus showing how this technology may be used as a tool to facilitate research.
Future extracellular matrix (ECM) platforms need to continue to improve in a number of ways. For example, while materials are evolutionarily optimized for the compatibility of cells with the cell-growth environment, it is unclear whether and how materials affect the activities of cellular components, most notably proteins. This manual presents work that seeks to determine how to more compositionally densely populate matrixes with engineered, therapeutic cells. d2c66b5586
Future extracellular matrix (ECM) platforms need to continue....